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    Question

    Which of the following is the correct sequence of

    reagents given below used in the Gram staining process? (A) lodine (B) Crystal violet (C) Safranın (D) Alcohol Choose the correct answer from the options given below:
    A (A), (B) (C) (D) Correct Answer Incorrect Answer
    B (A), (C), (B), (D) Correct Answer Incorrect Answer
    C (B), (A), (D), (C) Correct Answer Incorrect Answer
    D (C), (B), (D). (A) Correct Answer Incorrect Answer

    Solution

    The correct order of reagents in Gram staining is: (B) Crystal violet: Primary stain. (A) Iodine: Mordant, which fixes the dye to the cell wall. (D) Alcohol: Decolorizer that removes the primary dye from Gram-negative cells. (C) Safranin: Counterstain that stains Gram-negative cells pink.

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